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KMID : 1098420110190020103
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Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 2 p.103 ~ p.110
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Enhancement of Anticancer Activities from Lithospermum erythrorhizon Extracts by Ultra High Pressure Process
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Seo Yong-chang
Choi Woon-Yong
Kim Ji-Seon
Cho Jeong-Sub
Kim Young-Ock
Kim Jin-Chul
Lee Hyeon-Yong
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Abstract
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This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts () from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.
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KEYWORD
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Lithospermum erythrorhizon, Shikonin, Ultra High Pressure, Anticancer Activities
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